Price: USD 209 / kg
Min. Order: 1 kg
CAS: 8008-63-72
Content: 65%
This product since the cow or pig bile extract mixture of bile acid salts.
Description: light yellow or yellowish-brown powder;At the beginning of the smell of fresh bile smelly, sweet after bitter;Have led to wet.
It is easily dissolved in water or ethanol, insoluble in ether.
Application: biochemical reagent, also used in medicine.
Preparation: ox bile after enrichment with ethanol extraction, then through evaporation and ox-gall purification section.
Main ingredients: various ox-gall acid, mainly with the ammonia ox-gall acid sodium and taurocholic acid sodium.
Identification test:
1. Sodium test results (IT - 28).
2.1:10 ethanol liquid sample with iodine test solution (0.5 ml mixed TS - 124), should appear blue.
Content analysis:
(1) standard solution: accurately according to a certain amount of the United States pharmacopoeia (USP) bile acid reference standards, with 60% acetic acid standard solution preparation of 0.5 mg/ml.Such as cold storage can be used several months.
(2) sample preparation: about 50 mg, accurately said sample dissolved in 60% ethyl acid 100 ml, mix.At the beginning of filtering, abandon to filtrate 10 mg.
(3) operation: 1.0 ml, standard solution and sample solution into the binary high container respectively.In each container, add new preparation of 1:100 furfural liquid 1.0 ml.The container cooling in an ice bath for 5 min.Added by 50 ml you add 65 ml of water dilute sulfate sulfur acid and ml.Blend in 70 ℃ water bath for 10 min.Then immediately placed in an ice bath 2 min.LCM cuvette in the spectrophotometer is used to test the absorbance at 650 nm, and then press type calculation of bile acid content in the sample (mg) :
Packing: 25 kg cardboard barrels
Ox-gall extraction liquid preparation methods of (8008-63-7) :
Ox bile after enrichment with ethanol extraction, then through evaporation and ox-gall purification section.
Main ingredients:
Various ox-gall acid, mainly with the ammonia ox-gall acid sodium and taurocholic acid sodium.
Cap:
GMP;Cheese 0.002% (FDA, 2000).
Identification test:
1. Sodium test results (IT - 28).
2.1:10 ethanol liquid sample with iodine test solution (0.5 ml mixed TS - 124), should appear blue.
Content analysis:
(1) standard solution: accurately according to a certain amount of the United States pharmacopoeia (USP) bile acid reference standards, with 60% acetic acid standard solution preparation of 0.5 mg/ml.Such as cold storage can be used several months.
(2) sample preparation: about 50 mg, accurately said sample dissolved in 60% ethyl acid 100 ml, mix.At the beginning of filtering, abandon to filtrate 10 mg.
(3) operation: 1.0 ml, standard solution and sample solution into the binary high container respectively.In each container, add new preparation of 1:100 furfural liquid 1.0 ml.The container cooling in an ice bath for 5 min.Added by 50 ml you add 65 ml of water dilute sulfate sulfur acid and ml.Blend in 70 ℃ water bath for 10 min.Then immediately placed in an ice bath 2 min.LCM cuvette in the spectrophotometer is used to test the absorbance at 650 nm, and then press type calculation of bile acid content in the sample (mg) :
100 c (Au/As)
Type c - cholic acid in the reference standard solution concentration, mg/ml;
Au and As - the biggest absorbance of the sample and standard liquid.